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Crecencio Amaro Quiñones Narvaez
Rosario Salazar Minaya
Francisco Tongo Pizarro
Jesús Marino Gomez Miguel
Edilberto Cueva Pérez
Contexto: La conductancia estomática media el uso del agua, condicionando la restauración andino-amazónica según la estrategia funcional de cada especie. Objetivo: Evaluar si la eficiencia fotoquímica del fotosistema II (Fv/O) predice la transpiración (E) de forma diferencial entre Calycophyllum spruceanum (capirona, adquisitiva) y Dipteryx spp. (shihuahuaco, conservadora), y si la conductancia estomática (Gs) media dicha relación. Materiales y métodos: Diseño cuasi-experimental transversal con medidas repetidas en vivero de la Universidad Nacional Daniel Alcides Carrión (Oxapampa, ~1800 m s. n. m.): 54 plantones clonales (27 por especie) en tres momentos diurnos (N=162). Las variables fueron Fv/O (independiente), Gs (mediadora), E (dependiente) y especie (moderadora). El flujo analítico comprendió siete pasos: análisis factorial confirmatorio (CB-SEM, MLR), VIF/HTMT, regresión jerárquica OLS-HC3 (M1-M6), mediación bootstrap BCa (10 000 muestras), moderación con pendientes simples, cinco pruebas de robustez y fsQCA (umbral 0,80). Resultados: Gs y E difirieron entre especies con efectos grandes (Gs: t=9,65; p<0,001; d=1,52; E: t=7,52; p<0,001; d=1,18). El modelo completo explicó el 50,2 % de la varianza de E. La interacción Fv/O×especie (β=0,24; p<0,001) invirtió la pendiente: negativa no significativa en capirona (β=−0,07; p=0,156) y positiva significativa en shihuahuaco (β=+0,17; p<0,001). La mediación por Gs alcanzó el 49,8 %, sin carácter concluyente (IC 95 % [−0,005; 0,109]). Ninguna receta fsQCA alcanzó suficiencia (máximos 0,769 y 0,720). Conclusión: El acoplamiento fotoquímico-hídrico es especie-dependiente y estomáticamente condicionado; la selección de especies y el manejo hídrico en vivero condicionan el uso del agua más que la eficiencia aislada, con implicaciones para viverización y restauración andino-amazónica.
Context: Stomatal conductance mediates water use, conditioning Andean-Amazonian restoration according to each species' functional strategy. Objective: To evaluate whether photosystem II efficiency (Fv/O) predicts transpiration (E) differentially between Calycophyllum spruceanum (capirona, acquisitive) and Dipteryx spp. (shihuahuaco, conservative), and whether stomatal conductance (Gs) mediates this relationship. Materials and methods: A cross-sectional quasi-experimental design with repeated measures was conducted at the nursery of Universidad Nacional Daniel Alcides Carrión (Oxapampa, ~1800 m a.s.l.). Fifty-four clonal seedlings (27 per species) were measured at three time points (N=162). Variables were Fv/O (independent), Gs (mediator), E (dependent) and species (moderator). The seven-step pipeline comprised confirmatory factor analysis (CB-SEM, MLR), VIF/HTMT, hierarchical OLS regression with HC3 errors (M1-M6), BCa bootstrap mediation (10,000 samples), simple-slopes moderation, five robustness checks and fsQCA (0.80 threshold). Results: Gs and E differed between species with large effects (Gs: t=9.65; p<0.001; d=1.52; E: t=7.52; p<0.001; d=1.18). The full model explained 50.2% of variance in E. The Fv/O×species interaction was significant (β=0.24; p<0.001) and reversed the slope: negative and non-significant in capirona (β=−0.07; p=0.156) and positive and significant in shihuahuaco (β=+0.17; p<0.001). Mediation through Gs reached 49.8% of the total effect, without conclusive character (95% CI [−0.005, 0.109]). No fsQCA recipe reached sufficiency (maxima 0.769 and 0.720). Conclusion: Photochemical-water coupling is species-dependent and conditioned by stomatal regulation; species selection and nursery water management shape water use more than isolated efficiency, with implications for Andean-Amazonian nurseries and restoration

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Relación fotoquímica-hídrica en dos especies arbóreas amazónicas: papel estomático y especie. Alfa Revista de Investigación en Ciencias Agronómicas y Veterinarias [Internet]. 2026 Oct. 1 [cited 2026 Oct. 13];10(30):1-18. Available from: https://revistaalfa.org/index.php/revistaalfa/article/view/710
Referencias

Cong W, Li X, Yang K, Li Y, Jin C, Lu S, Wang F. Quantifying PSII open centers and multiscale photosynthetic electron transport with solar-induced chlorophyll fluorescence in evergreen forests. Physiologia Plantarum. 2025;177. https://doi.org/10.1111/ppl.70687

Ziccardi L, Kramer D, Gonçalves N, Nelson B, Taylor T, Albert L, Campos K, Prohaska N, Restrepo N, Saleska S, Stark S. Seasonal and intracanopy shifts in the fates of absorbed photons in central Amazonian forests: implications for leaf fluorescence and photosynthesis. New Phytol. 2025; 248:76-91. https://doi.org/10.1111/nph.70183

Csillik O, Keller M, Longo M, Ferraz A, Pinagé E, Görgens E, Ometto J, Silgueiro V, Brown D, Duffy P, Cushman K, Saatchi S. A large net carbon loss attributed to anthropogenic and natural disturbances in the Amazon Arc of Deforestation. Proc Natl Acad Sci U S A. 2024;121. https://doi.org/10.1073/pnas.2310157121

Bourgoin C, Ceccherini G, Girardello M, Vancutsem C, Avitabile V, Beck P, Beuchle R, Blanc L, Duveiller G, Migliavacca M, Vieilledent G, Cescatti A, Achard F. Human degradation of tropical moist forests is greater than previously estimated. Nature. 2024;631:570-576. https://doi.org/10.1038/s41586-024-07629-0

Flores B, Montoya E, Sakschewski B, Nascimento N, Staal A, Betts R, Levis C, Lapola D, Esquível A, Jakovac C, Nobre C, Oliveira R, Borma L, Nian D, Boers N, Hecht S, Ter H, Arieira J, Lucas I, Berenguer E, Marengo J, Gatti L, Mattos C, Hirota M. Critical transitions in the Amazon forest system. Nature. 2024;626:555-564. https://doi.org/10.1038/s41586-023-06970-0

Longo M, Keller M, Kueppers L, Bowman K, Csillik O, Ferraz A, Moorcroft P, Ometto J, Soares B, Xu X, De M, Görgens E, Larson E, Needham J, Ordway E, Pereira F, Pinagé E, Sato L, Xu L, Saatchi S. Degradation and deforestation increase the sensitivity of the Amazon Forest to climate extremes. Environ Res Lett. 2025;20. https://doi.org/10.1088/1748-9326/adc58c

Martin S. Regeneration of secondary forest following anthropogenic disturbance from 1985 to 2021 for Amazonas, Brazil. Glob Chang Biol. 2024;30. https://doi.org/10.1111/gcb.17514

Mataveli G, Maure L, Sánchez A, Dutra D, De G, Jones M, Amaral C, Artaxo P, Aragão L. Forest degradation is undermining progress on deforestation in the Amazon. Glob Chang Biol. 2025;31. https://doi.org/10.1111/gcb.70209

Faitanin M, De C, Villela D. A background about leaf economic spectrum in tropical vegetation. ERR01. 2026. https://doi.org/10.56238/err01v11n1-003

Ziccardi L, Taylor T, Smith M, Gonçalves N, Kramer D, Campos K, Prohaska N, Restrepo N, Saleska S, Siqueira A, Penha D, Gomez R, Santos V, Stark S. Trait coordination reveals the fast–slow plant economics spectrum along the vertical canopy profile in central Amazonian forests. Funct Ecol. 2025. https://doi.org/10.1111/1365-2435.70213

Yu L, Luo X, Croft H, Rogers C, Chen J. Seasonal variation in the relationship between leaf chlorophyll content and photosynthetic capacity. Plant Cell Environ. 2024. https://doi.org/10.1111/pce.14997

Nyongesa J, Oronyi W, Lawrence O, Ronoh E, Mwalati L, Suba V, Gayo L, Nkengurutse J, Otieno D, Li Y. Seasonal variation in transpiration and stomatal conductance of three savanna tree species in Ruma National Park, Kenya. Forests. 2025. https://doi.org/10.3390/f16060999

Diao H, Cernusak L, Saurer M, Gessler A, Siegwolf R, Lehmann M. Uncoupling of stomatal conductance and photosynthesis at high temperatures: mechanistic insights from online stable isotope techniques. New Phytol. 2024. https://doi.org/10.1111/nph.19558

Chaturvedi R, Pandey S, Tripathi A, Goparaju L, Singh A, Singh J, López L, Bargali S. Variations in the plasticity of functional traits indicate the differential impacts of abiotic and biotic factors on the structure and growth of trees in tropical dry forest fragments. Front Plant Sci. 2024;14. https://doi.org/10.3389/fpls.2023.1181293

Chaturvedi R, Tomlinson K, Pandey S, Tripathi A, Raghubanshi A, Singh J. Changes in plant traits and productivity of two functional types across a soil water content gradient in a tropical dry forest. J Ecol. 2025;113:3535-3549. https://doi.org/10.1111/1365-2745.70177

Vega F, Cifuentes L, Pineda F, Dawson T, Paz H. Different dry-wet pulses favor different functional strategies: a test using tropical dry forest tree species. PLoS One. 2024;19. https://doi.org/10.1371/journal.pone.0309510

Ziegler C, Cochard H, Stahl C, Foltzer L, Gérard B, Goret J, Heuret P, Levionnois S, Maillard P, Bonal D, Coste S. Residual water losses mediate the trade-off between growth and drought-survival across saplings of 12 tropical rainforest tree species with contrasting hydraulic strategies. J Exp Bot. 2024. https://doi.org/10.1093/jxb/erae159

Medina J, Montalván E, Pierick K, Benítez Á, Cumbicus N, Homeier J. Anatomical variation in root traits reflects the continuum from slow to fast growth strategies among tropical tree species. Plants. 2025;14. https://doi.org/10.3390/plants14233590

Wang T, Dong C, Wan H, Zou R, Ren R, Yu S, Xie X. Coordination between water source partitioning and isohydric-anisohydric behavior shapes contrasting water use strategies of Tamarix chinensis and Ulmus pumila in saline-alkaline soils. Front Plant Sci. 2025;16. https://doi.org/10.3389/fpls.2025.1697666

Yang J, Lu X, Liu Z, Tang X, Yu Q, Wang Y. Atmospheric drought dominates changes in global water use efficiency. Sci Total Environ. 2024. https://doi.org/10.1016/j.scitotenv.2024.173084

Hu M, Sun S, Cheng X, Pan Q, Zhang J, Wang X, Guan C, Li Z, Gao X. Specific responses to environmental factors cause discrepancy in the link between solar-induced chlorophyll fluorescence and transpiration in three plantations. Remote Sens. 2025;17. https://doi.org/10.3390/rs17091625

Brum M, Vadeboncoeur MA, Asbjornsen H, Puma Vilca BL, Galiano D, Horwath AB, et al. Ecophysiological controls on water use of tropical cloud forest trees in response to experimental drought. Tree Physiol. 2023;43(9):1514-1532. https://doi.org/10.1093/treephys/tpad070

Bravo-Avila CH, Feeley KJ. Variation in the drought tolerance of tropical understory plant communities across an extreme elevation and precipitation gradient. Plants. 2023;12(16):2957. https://doi.org/10.3390/plants12162957

Macancela-Herrera A, Smith R. Stomatal morphometry of Andean species and their relationship with spatial variation. iForest. 2025;18(6):327-334. https://doi.org/10.3832/ifor4689-018.

Busch F, Ainsworth E, Amtmann A, Cavanagh A, Driever S, Ferguson J, Kromdijk J, Lawson T, Leakey A, Matthews J, Meacham K, Vath R, Vialet S, Walker B, Papanatsiou M. A guide to photosynthetic gas exchange measurements: fundamental principles, best practice and potential pitfalls. Plant Cell Environ. 2024. https://doi.org/10.1111/pce.14815

Oivukkamäki J, Aalto J, Pfündel E, Tian M, Zhang C, Grebe S, Salmon Y, Hölttä T, Porcar A. Field integration of shoot gas-exchange and leaf chlorophyll fluorescence measurements to study the long-term regulation of photosynthesis in situ. Tree Physiol. 2024;45. https://doi.org/10.1093/treephys/tpae162

Kimura K, Fushimi E, Kumagai E, Nomura K, Matsunami T, Konno S, Maruyama A. Estimating leaf CO2 assimilation in C3 plants using a handheld porometer with chlorophyll fluorometer in field conditions. Plant Cell Environ. 2025;48:7213-7224. https://doi.org/10.1111/pce.70006

Pappert I, Klir S, Jokic L, Ühlein C, Quoc K, Kaldenhoff R. Plant photosynthesis in basil (C3) and maize (C4) under different light conditions as basis of an AI-based model for PAM fluorescence/gas-exchange correlation. Front Plant Sci. 2025;16. https://doi.org/10.3389/fpls.2025.1590884

Wang Z, Slot M, Wang C. Decoupling of stomatal conductance, transpiration and photosynthesis in terrestrial plants under elevated temperature: a meta-analysis. Nat Commun. 2026;17. https://doi.org/10.1038/s41467-025-68250-x

Zheng C, Wang S, Chen J, Xiao J, Chen J, Zhu K, Sun L. Modeling transpiration using solar-induced chlorophyll fluorescence and photochemical reflectance index synergistically in a closed-canopy winter wheat ecosystem. Remote Sens Environ. 2024. https://doi.org/10.1016/j.rse.2023.113981

Zhang Y, Cai M, Xiao X, Yang X, Migliavacca M, Basara J, Zhou S, Deng Y. Immediate and lagged vegetation responses to dry spells revealed by continuous solar-induced chlorophyll fluorescence observations in a tall-grass prairie. Remote Sens Environ. 2024. https://doi.org/10.1016/j.rse.2024.114080

Wittemann M, Mujawamariya M, Ntirugulirwa B, Uwizeye F, Zibera E, Manzi O, Nsabimana D, Wallin G, Uddling J. Plasticity and implications of water-use traits in contrasting tropical tree species under climate change. Physiol Plant. 2024;176. https://doi.org/10.1111/ppl.14326

Palomo J, Valdez M, Islebe G, Osorio E, Cruz G, López F, Juárez R. Hydro-functional strategies of sixteen tree species in a Mexican karstic seasonally dry tropical forest. Forests. 2025. https://doi.org/10.3390/f16101535

Yan W, Lu Y, Guo L, Liu Y, Li M, Zhang B, Zhang B, Zhang L, Qin D, Huo J. Effects of drought stress on photosynthesis and chlorophyll fluorescence in blue honeysuckle. Plants. 2024;13. https://doi.org/10.3390/plants13152115