Prevalencia de Staphylococcus aureus en manipuladores de alimentos en restaurantes de Lima
Prevalence of Staphylococcus aureus among food handlers in restaurants in LimaContenido principal del artículo
Contexto: Las enfermedades transmitidas por alimentos son una prioridad en salud pública mundial, especialmente en servicios de alimentación colectiva, debido al riesgo de brotes y afectación de la población. Objetivo: Determinar la prevalencia de Staphylococcus aureus en manipuladores de alimentos de restaurantes del distrito de Independencia, Lima, Perú. Metodología: Se realizó un estudio cuantitativo, no experimental, transversal y descriptivo. La muestra incluyó 450 manipuladores de 170 restaurantes, seleccionados mediante muestreo probabilístico estratificado. Se recolectaron muestras por enjuague de manos y se procesaron en agar Baird-Parker para identificar la presencia de S. aureus. Resultados: Se detectó que 136 manipuladores superaron los límites microbiológicos, representando una prevalencia de 30,2 %. La distribución de casos fue homogénea entre las distintas zonas geográficas evaluadas. Conclusiones: Existe una elevada prevalencia de S. aureus en manipuladores de alimentos en restaurantes del distrito de Independencia, lo que constituye un riesgo potencial para la inocuidad alimentaria. Se recomienda fortalecer las medidas de vigilancia sanitaria y promover programas de capacitación en manipulación segura de alimentos para reducir la exposición y prevenir enfermedades transmitidas por alimentos.
Context: Foodborne diseases (FBDs) represent a critical global public health priority, particularly within collective catering services, where the risk of outbreaks poses a significant threat to population health. Objective: To determine the prevalence of Staphylococcus aureus among food handlers in restaurants located in the Independencia district of Lima, Peru. Methodology: A quantitative, non-experimental, cross-sectional, and descriptive study was conducted. The sample comprised 450 food handlers from 170 restaurants, selected via stratified random sampling. Samples were collected using the hand rinse technique and processed on Baird-Parker agar for the isolation and identification of S. aureus. Results: Laboratory analysis revealed that 136 food handlers exceeded established microbiological limits, resulting in a 30.2% prevalence of S. aureus. The distribution of positive cases was homogeneous across the different geographical zones evaluated. Conclusions: The high prevalence of S. aureus among food handlers in the Independencia district signifies a substantial risk to food safety. These findings underscore the urgent need to strengthen sanitary surveillance and implement comprehensive training programs in safe food handling to mitigate contamination risks and prevent foodborne illnesses.
Descargas
Detalles del artículo
Beyene G, Mamo G, Kassa T, Tasew G, Mereta ST. Nasal and Hand Carriage Rate of Staphylococcus aureus among Food Handlers Working in Jimma Town, Southwest Ethiopia. Ethiop J Health Sci. 2019;29(5):605-12. https://doi.org/10.4314/ejhs.v29i5.11
Nasrolahei M, Mirshafiee S, Kholdi S, Salehian M, Nasrolahei M. Bacterial assessment of food handlers in Sari City, Mazandaran Province, north of Iran. J Infect Public Health. 2017;10(2):171-6. https://www.sciencedirect.com/science/article/pii/S1876034116300272
da Silva SDSP, Cidral TA, Soares MJ dos S, de Melo MCN. Enterotoxin-Encoding Genes in Staphylococcus spp. from Food Handlers in a University Restaurant. Foodborne Pathog Dis. 2015;12(11):921-5. https://doi.org/10.1089/fpd.2015.1941
Gündüz T, Limoncu ME, Cümen S, Ari A, Serdağ E, Tay Z. The prevalence of intestinal parasites and nasal S. aureus carriage among food handlers. J Environ Health. 2008;70(10):64-5, 67. https://pubmed.ncbi.nlm.nih.gov/18561571/
Baptistão LG, Silva NCC, Bonsaglia ECR, Rossi BF, Castilho IG, Fernandes Junior A, et al. Presence of Immune Evasion Cluster and Molecular Typing of Methicillin-Susceptible Staphylococcus aureus Isolated from Food Handlers. J Food Prot. 2016;79(4):682-6. https://doi.org/10.4315/0362-028X.JFP-15-401
Romano A, Carrella S, Rezza S, Nia Y, Hennekinne JA, Bianchi DM, et al. First Report of Food Poisoning Due to Staphylococcal Enterotoxin Type B in Döner Kebab (Italy). Pathogens. 2023;12(9):1139. https://doi.org/10.3390/pathogens12091139
Ercoli L, Gallina S, Nia Y, Auvray F, Primavilla S, Guidi F, et al. Investigation of a Staphylococcal Food Poisoning Outbreak from a Chantilly Cream Dessert, in Umbria (Italy). Foodborne Pathog Dis. 2017;14(7):407-13. https://doi.org/10.1089/fpd.2016.2267
Isara AR, Isah EC, Lofor PVO, Ojide CK. Food contamination in fast food restaurants in Benin City, Edo State, Nigeria: Implications for food hygiene and safety. Public Health. 2010;124(8):467-71. https://doi.org/10.1016/j.puhe.2010.03.028
Moumni HA, Dahbi I, Akrim M, Meski FZ, Khader Y, Lakranbi M, et al. Outbreak Investigation of a Multipathogen Foodborne Disease in a Training Institute in Rabat, Morocco: Case-Control Study. JMIR Public Health Surveill. 2019;5(3):e14227. https://doi.org/10.2196/14227
Lynch B, Sepúlveda A, Lillo R, Diaz MC, Dinamarca O. Carriers of Staphylococcus aureus among food handlers. Rev Med Chil. 1974;102(4):277-8.
Al Bustan MA, Udo EE, Chugh TD. Nasal carriage of enterotoxin-producing Staphylococcus aureus among restaurant workers in Kuwait City. Epidemiol Infect. 1996;116(3):319-22. https://www.cambridge.org/core/journals/epidemiology-and-infection/article/nasal-carriage-of-enterotoxinproducing-staphylococcus-aureus-among-restaurant-workers-in-kuwait-city/8F35CCAF53D4019CB3FF38B7377BA333
Asghar A, Zafar T, Momenah A. Bacteriological and serological survey of infectious diseases among food handlers in Makkah. Ann Saudi Med. 2006;26(2):141-4. https://doi.org/10.5144/0256-4947.2006.141
Okafor UC, Okorie-Kanu OJ, Ogugua AJ, Ikeogu CF, Okafor SC, Anyanwu MU, et al. Molecular Epidemiology, Antimicrobial Resistance, and Virulence Profiles of Staphylococcus aureus from Fish, Aquatic Environments, and Fish Handlers in Southeast Nigeria. Microorganisms. 2025;13(9):2059. https://doi.org/10.3390/microorganisms13092059
Pakbin B, Amani Z, Rahimi Z, Najafi S, Familsatarian B, Khakpoor A, et al. Prevalence of Foodborne Bacterial Pathogens and Antibiotic Resistance Genes in Sweets from Local Markets in Iran. Foods. 2023;12(19):3645. https://doi.org/10.3390/foods12193645
Fernandes A, Ramos C, Monteiro V, Santos J, Fernandes P. Virulence Potential and Antibiotic Susceptibility of S. aureus Strains Isolated from Food Handlers. Microorganisms. 2022;10(11):2155. https://doi.org/10.3390/microorganisms10112155
Parvin MS, Ali MY, Talukder S, Nahar A, Chowdhury EH, Rahman MT, et al. Prevalence and Multidrug Resistance Pattern of Methicillin Resistant S. aureus Isolated from Frozen Chicken Meat in Bangladesh. Microorganisms. 2021;9(3):636. https://doi.org/10.3390/microorganisms9030636
Aung MS, San T, Aye MM, Mya S, Maw WW, Zan KN, et al. Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar. Toxins. 2017;9(8):241. https://doi.org/10.3390/toxins9080241
Udo EE, Al-Mufti S, Albert MJ. The prevalence of antimicrobial resistance and carriage of virulence genes in Staphylococcus aureus isolated from food handlers in Kuwait City restaurants. BMC Res Notes. 2009;2:108. https://doi.org/10.1186/1756-0500-2-108
Tasanapak K, Kucharoenphaibul S, Wongwigkarn J, Sitthisak S, Thummeepak R, Chaibenjawong P, et al. Prevalence and virulence genes of Staphylococcus aureus from food contact surfaces in Thai restaurants. PeerJ. 2023;11:e15824. https://peerj.com/articles/15824/
Eke SO, Eloka CCV, Mgbachi N, Nwobodo HA, Ekpen-Itamah UJ. Nasal carriage of Staphylococcus aureus among food handlers and restaurant workers in Ekpoma Edo state, Nigeria. Int J Community Res. 2015;4(1):7-14. https://www.ajol.info/index.php/ijcr/article/view/124927
Aubed MH, Almeahy AW, Jabbar MR, Shahib NA, Maktouf HH, Hamid B. Determining the Prevalence of Staphylococcus aureus Bacteria in Nasal Swabs from Restaurant Workers in Al-Kut City. Cent Asian J Theor Appl Sci. 2025;6(4):626-32. https://cajotas.casjournal.org/index.php/CAJOTAS/article/view/1596
Abdulrahman MA, Taher AI. Prevalence of methicillin resistant Staphylococcus aureus among food handlers in Duhok City. Sci J Univ Zakho. 2018;6(4):140-5. http://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/540
Angkititrakul S, Polpakdee A, Chuanchuen R. Prevalence of Salmonella enterica, Escherichia coli and Staphylococcus aureus in raw meat in Thai self-service style restaurants in Khon kaen municipality. Thai J Vet Med. 2013;43(2):265-8. https://digital.car.chula.ac.th/tjvm/vol43/iss2/13/
Saeed HA, Hamid HH. Bacteriological and parasitological assessment of food handlers in the Omdurman area of Sudan. J Microbiol Immunol Infect Wei Mian Yu Gan Ran Za Zhi. 2010;43(1):70-3. https://doi.org/10.1016/S1684-1182(10)60010-2
Tan E. Towards a managerial state: Turkey’s decentralization reforms under the AKP government. En: Public Sector Reforms in Developing Countries. Routledge; 2014. p. 124-38. https://www.taylorfrancis.com/chapters/edit/10.4324/9780203797587-12/towards-managerial-state-evrim-tan
Seow WL, Mahyudin NA, Amin-Nordin S, Radu S, Abdul-Mutalib NA. Antimicrobial resistance of Staphylococcus aureus among cooked food and food handlers associated with their occupational information in Klang Valley, Malaysia. Food Control. 2021;124:107872. https://www.sciencedirect.com/science/article/pii/S0956713521000104
Bukhari MA, Banasser TM, El-Bali M, Bulkhi RA, Qamash RA, Trenganno A, et al. Assessment of microbiological quality of food preparation process in some restaurants of Makkah city. Saudi J Biol Sci. 2021;28(10):5993-7. https://www.sciencedirect.com/science/article/pii/S1319562X21005271
Castro A, Santos C, Meireles H, Silva J, Teixeira P. Food handlers as potential sources of dissemination of virulent strains of Staphylococcus aureus in the community. J Infect Public Health. 2016;9(2):153-60. https://www.sciencedirect.com/science/article/pii/S1876034115001604
Ayele MT, Berihun AM, Worku AK, Getnet MA. Isolation, Antibiogram and Factors Associated With Staphylococcus aureus From Fish at the Landing Site and Selected Restaurants in Central Gondar, Ethiopia: One Health Approach. Vet Med Sci. 2026;12(2):e70880. https://doi.org/10.1002/vms3.70880
Ncube F, Kanda A, Chijokwe M, Mabaya G, Nyamugure T. Food safety knowledge, attitudes and practices of restaurant food handlers in a lower‐middle‐income country. Food Sci Nutr. 2020;8(3):1677-87. https://doi.org/10.1002/fsn3.1454
Sospedra I, Mañes J, Soriano JM. Report of toxic shock syndrome toxin 1 (TSST-1) from Staphylococcus aureus isolated in food handlers and surfaces from foodservice establishments. Ecotoxicol Environ Saf. 2012;80:288-90. https://www.sciencedirect.com/science/article/pii/S014765131200098X
Dagnew M, Tiruneh M, Moges F, Tekeste Z. Survey of nasal carriage of Staphylococcus aureus and intestinal parasites among food handlers working at Gondar University, Northwest Ethiopia. BMC Public Health. 2012;12(1):837. https://doi.org/10.1186/1471-2458-12-837
Balzaretti CM, Marzano MA. Prevention of travel-related foodborne diseases: Microbiological risk assessment of food handlers and ready-to-eat foods in northern Italy airport restaurants. Food Control. 2013;29(1):202-7. https://www.sciencedirect.com/science/article/pii/S0956713512003490
Lynch RA, Phillips ML, Elledge BL, Hanumanthaiah S, Boatright DT. A preliminary evaluation of the effect of glove use by food handlers in fast food restaurants. J Food Prot. 2005;68(1):187-90. https://www.sciencedirect.com/science/article/pii/S0362028X22008924